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Cas9 gRNA engineering for genome editing, activation and repression.
Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR, Collins JJ, Weiss R, Church G
Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
68495AAV_NLS-dSaCas9-NLS-VPRAAV vector containing nuclease null SaCas9 fused to VPR
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68496AAV_NLS-SaCas9-NLS-VPRAAV vector containing SaCas9 fused to VPR
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68497hCas9-VPRnuclease competent SP-Cas9 fused to VPR
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68498M-ST1-VPRnuclease competent ST1-Cas9 fused to VPR
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79368gRNA_tdtomato_reporter_1_ST1_2x_bindingAssays activity of transcriptional activators fused to ST1 Cas9
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79369gRNA_tdtomato_reporter_1_SA-2xAssays activity of transcriptional activators fused to SA Cas9
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79370gRNA_tdtomato_reporter1_SP-2xAssays activity of transcriptional activators fused to SP Cas9
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Antibodies from Article