An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis.
Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM
Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016. PubMed Article
Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM
Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 72872 | PMXS-GOT1 | The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly. |
| 72873 | PMXS-SLC1A3 | The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly |
| 72874 | plentiCRISPR-sgGOT1 | The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB). |
| 72875 | plentiCRISPR-sgMDH1 | The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB |
| 110066 | Human CRISPR Metabolic Gene Knockout Library | Encodes gRNAs for targeting metabolic genes in CRISPR screens. |