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Dissecting the roles of human BUB1 in the spindle assembly checkpoint.
Vleugel M, Hoek TA, Tromer E, Sliedrecht T, Groenewold V, Omerzu M, Kops GJ
J Cell Sci. 2015 Aug 15;128(16):2975-82. doi: 10.1242/jcs.169821. Epub 2015 Jul 6. PubMed Article

Plasmids from Article

ID Plasmid Purpose
115903pcDNA5-LAP-BUB1Used for expression of siRNA-resistant BUB1-WT from the FRT site of FlpIn cells upon addition of doxycyclin.
115904pcDNA5-MIS12-LAP-BUB1Used for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.
115905pLacI-LAP-BUB1Used to express BUB1-WT fused to LacI for recruitment of BUB1 to LacO spots.

Antibodies from Article