A fluorescent tool set for yeast Atg proteins.
Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, Xie Z
Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971. PubMed Article
Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, Xie Z
Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69160 | LD101 | Integration of GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69161 | LD102 | Integration of GFP tag at Atg2 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69162 | LD105 | Integration of GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69163 | LD106 | Integration of GFP tag at Atg6 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69164 | LD109 | Integration of GFP tag at Atg9 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69165 | LD111 | Integration of GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69166 | LD113 | Integration of GFP tag at Atg13 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69167 | LD114 | Integration of GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69168 | LD116 | Integration of GFP tag at Atg16 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69169 | LD117 | Integration of GFP tag at Atg17 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69170 | LD118 | Integration of GFP tag at Atg18 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69171 | LD120 | Integration of GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69172 | LD121 | Integration of GFP tag at Atg21 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69173 | LD123 | Integration of GFP tag at Atg23 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69174 | LD124 | Integration of GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69175 | LD127 | Integration of GFP tag at Atg27 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69176 | LD129 | Integration of GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69177 | LD131 | Integration of GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69178 | LD201 | Integration of 2GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69179 | LD202 | Integration of 2GFP tag at Atg2 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69180 | LD205 | Integration of 2GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69181 | LD206 | Integration of 2GFP tag at Atg6 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69182 | LD209 | Integration of 2GFP tag at Atg9 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69183 | LD211 | Integration of 2GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69184 | LD213 | Integration of 2GFP tag at Atg13 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69185 | LD214 | Integration of 2GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69186 | LD217 | Integration of 2GFP tag at Atg17 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69187 | LD218 | Integration of 2GFP tag at Atg18 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69188 | LD220 | Integration of 2GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69189 | LD221 | Integration of 2GFP tag at Atg21 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69190 | LD223 | Integration of 2GFP tag at Atg23 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69191 | LD224 | Integration of 2GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69192 | LD229 | Integration of 2GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69193 | LD231 | Integration of 2GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans) |
| 69194 | BS-Ura3-GFP-Atg8 | Single copy integration of GFP-Atg8, use auxotrophic marker URA3(Kluyveromyces lactis) |
| 69195 | BS-Ura3Kl | complementation of yeast auxotrophic marker URA3(Kluyveromyces lactis) |
| 69196 | BS-Trp1 | complementation of yeast auxotrophic marker TRP1. Although this is the plasmid described in the paper, the depositor notes that it has a low integration efficiency and Plasmid 69503 should be used. |
| 69197 | BS-His3 | complementation of yeast auxotrophic marker HIS3 |
| 69198 | BS-Met15 | complementation of yeast auxotrophic marker MET15 |
| 69199 | BS-Lys2 | complementation of yeast auxotrophic marker LYS2 |
| 69200 | BS-Leu2 | complementation of yeast auxotrophic marker LEU2 |
| 69201 | PCUP1-DuDre-Atg8-404 | RFP-Atg8 (PAS marker) with improved property |
| 69503 | LD-BS-Trp1 | Improved version for complementation of yeast auxotrophic marker TRP1 |