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Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 from Its Activation.
Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. Epub 2015 May 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
71724pcDNA-hHSF1 WTplasmid expressing myc-his tag hHSF1 WT
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71725pcDNA-HSF1Δ∼PRDplasmid expressing mic-his tag phosphorylation-deficient hHSF1
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71726Gal4-VP16-HSF1 WTplasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD
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71727Gal4-VP16-HSF1Δ∼PRDplasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RD
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71728Gal4-VP16plasmid expressing fusion construct of Gal4 DBD and VP16 AD
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Antibodies from Article