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Stem cells. Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness.
Katajisto P, Dohla J, Chaffer CL, Pentinmikko N, Marjanovic N, Iqbal S, Zoncu R, Chen W, Weinberg RA, Sabatini DM
Science. 2015 Apr 17;348(6232):340-3. doi: 10.1126/science.1260384. Epub 2015 Apr 2. PubMed Article

Plasmids from Article

ID Plasmid Purpose
69598paGFP-Omp25expresses GFP-labeled mitochondrial outer membrane protein 25
69599Snap-Omp25Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

Antibodies from Article