Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. PubMed Article
Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64215 | pAAVS1-TLR targeting vector | Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct |
| 64216 | pU6-sgRosa26-1_CBh-Cas9-T2A-BFP | Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide |
| 64217 | pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI) | Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A |
| 64218 | pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B | Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A |
| 64219 | pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B | Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A |
| 64220 | pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6 | Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A |
| 64221 | pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B | Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2A |
| 64222 | pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6 | Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A |
| 64322 | pTLR repair vector | Vector for repair of the traffic light reporter (TLR), providing the venus coding region |
| 64323 | pU6-(BbsI)_CBh-Cas9-T2A-BFP | Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide |
| 64324 | pU6-(BbsI)_CBh-Cas9-T2A-mCherry | Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide |