Highly efficient Cas9-mediated transcriptional programming.
Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM
Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. PubMed Article
Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM
Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63798 | SP-dCas9-VPR | SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector |
| 63799 | M_ST1n_VPR | ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector |
| 63800 | PB-TRE-dCas9-VPR | SP-dCas9-VPR with doxycycline-inducible expression |
| 63801 | pAG414GPD-dCas9-VPR | pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector |
| 63802 | pAWG-dCas9-VPR | SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector |
| 64046 | pSB700 | Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein |