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Multicolor CRISPR labeling of chromosomal loci in human cells.
Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
64104pHAGE-EFS-dCas9-GFPExpression of Sp dCas9-GFP in mammalian cells
64105pHAGE-SSFV-dCas9-GFPExpression of Sp dCas9-GFP in mammalian cells
64106pHAGE-TO-dCas9-GFPExpression of Sp dCas9-GFP in mammalian cells
64107pHAGE-TO-dCas9-3XGFPExpression of Sp dCas9-3XGFP in mammalian cells
64108pHAGE-TO-dCas9-3XmCherryExpression of Sp dCas9-3XmCherry in mammalian cells
64109pHAGE-TO-nmdCas9-3XGFPExpression of Nm dCas9-3XGFP in mammalian cells
64110pHAGE-TO-nmdCas9-3XmCherryExpression of Nm dCas9-3XmCherry in mammalian cells
64111pHAGE-TO-nls-st1dCas9-3nls-3XGFPExpression of St1 dCas9-3XGFP in mammalian cells
64112pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nlsExpression of St1 dCas9-3XGFP in mammalian cells
64113pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nlsExpression of St1 dCas9-3XGFP in mammalian cells
64114pLH-spsgRNA2Vector for expression of Sp sgRNA2 in mammalian cells
64115pLH-nmsgRNA1.1Vector for expression of Nm sgRNA1.1 in mammalian cells
64116pLH-stsgRNA1.1Vector for expression of St1 sgRNA1.1 in mammalian cells
64117pLH-stsgRNA2.1Vector for expression of St1 sgRNA2.1 in mammalian cells
64118pLH-stsgRNA3.1Vector for expression of St1 sgRNA3.1 in mammalian cells
64512pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2Expression of St1 dCas9-3XTagBFP2 in mammalian cells

Antibodies from Article