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Fixation-resistant photoactivatable fluorescent proteins for CLEM.

Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, Wang Y, Looger LL
Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
51072pRSETa_ mEos4aPhotoconverible Fluorescent Protein_ Fixative Resistant
51073pRSETa_ mEos4bPhotoconverible Fluorescent Protein_ Fixative Resistant (monomeric)
54810mEos4a-C1Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
54811mEos4a-N1Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
54812mEos4b-C1Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
54813mEos4b-Lifeact-7Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581
54814mEos4b-N1Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Antibodies from Article