Dynamic visualization of alpha-catenin reveals rapid, reversible conformation switching between tension states.
Kim TJ, Zheng S, Sun J, Muhamed I, Wu J, Lei L, Kong X, Leckband DE, Wang Y
Curr Biol. 2015 Jan 19;25(2):218-24. doi: 10.1016/j.cub.2014.11.017. Epub 2014 Dec 24. PubMed Article
Kim TJ, Zheng S, Sun J, Muhamed I, Wu J, Lei L, Kong X, Leckband DE, Wang Y
Curr Biol. 2015 Jan 19;25(2):218-24. doi: 10.1016/j.cub.2014.11.017. Epub 2014 Dec 24. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71709 | alpha catenin conformation sensor | This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation. |
| 71710 | VBS mutant of alpha catenin sensor | This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin. |
| 71711 | beta catenin binding mutant of alpha catenin sensor | This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions |
| 198245 | α-catenin ECFP-YPET FRET ΔF-actin binding site | Alpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin. |