Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Nielsen AA, Voigt CA
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735. PubMed
Nielsen AA, Voigt CA
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62244 | pAN-PTet-dCas9 | controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter. |
| 62245 | pAN-PTet-YFP | used to characterize the promoter activities of PTet for dCas9 and sgRNA response functions. |
| 62246 | pAN-PBAD-YFP | used to characterize the promoter activities of PBAD for dCas9 and sgRNA response functions. |
| 62247 | pAN-PA1-RFP | reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1 |
| 62248 | pAN-PA2-RFP | reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1 |
| 62249 | pAN-PA3-RFP | reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1 |
| 62250 | pAN-PA4-RFP | reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1 |
| 62251 | pAN-PA5-RFP | reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1 |
| 62255 | pAN-PBAD-sgRNA-A1NT | expression of A1NT sgRNA from the arabinose-inducible promoter |
| 62256 | pAN-PBAD-sgRNA-A1T | expression of A1T sgRNA from the arabinose-inducible promoter |
| 62257 | pAN-PBAD-sgRNA-A2NT | expression of A2NT sgRNA from the arabinose-inducible promoter |
| 62258 | pAN-PBAD-sgRNA-A2T | expression of A2T sgRNA from the arabinose-inducible promoter |
| 62259 | pAN-PBAD-sgRNA-A3NT | expression of A3NT sgRNA from the arabinose-inducible promoter |
| 62260 | pAN-PBAD-sgRNA-A3T | expression of A3T sgRNA from the arabinose-inducible promoter |
| 62261 | pAN-PBAD-sgRNA-A4NT | expression of A4NT sgRNA from the arabinose-inducible promoter |
| 62262 | pAN-PBAD-sgRNA-A4T | expression of A4T sgRNA from the arabinose-inducible promoter |
| 62263 | pAN-PBAD-sgRNA-A5NT | expression of A5NT sgRNA from the arabinose-inducible promoter |
| 62264 | pAN-PBAD-sgRNA-A5T | expression of A5T sgRNA from the arabinose-inducible promoter |
| 62285 | pAN-PBAD-sgRNA-scramble | expression of scramble sgRNA from the arabinose-inducible promoter |
| 62286 | pAN-PBAD-sgRNA-VR | expression of VR sgRNA from the arabinose-inducible promoter |
| 62306 | pAN-NOR | synthetic circuit: NOR gate |
| 62307 | pAN-OR | synthetic circuit: OR gate |
| 62308 | pAN-AND | synthetic circuit: AND gate |
| 62309 | pAN-OR-MalT-3NT | synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome |