Addgene: The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny.

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Holger Puchta
Schiml et al

The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny.
Schiml S, Fauser F, Puchta H
Plant J. 2014 Oct 18. doi: 10.1111/tpj.12704. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
61479	pEn-C1.1	Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

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