A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging.
Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. PubMed Article
Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60903 | pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP | Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation |
| 60904 | pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS | The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64 |
| 60905 | pLV-sgCDKN1B#2 BFP | A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP |
| 60906 | pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE | The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging |
| 60907 | pHR-scFv-GCN4-sfGFP-GB1-dWPRE | The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging |
| 60909 | pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro | A minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imaging |
| 60910 | pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE | dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system |
| 60911 | pcDNA4TO-H2B-SunTag24x_v1 | Enocdes a histone H2B fused to the SunTag for single molecule imaging |
| 60912 | pcDNA4TO-CAMSAP1L1-24xGCN4-IRES-puro | Enocdes Camsap1L1 fused to the SunTag for single molecule imaging |
| 60913 | pcDNA4TO-mito-mCherry-24xGCN4_v1 | A fusion protein of the SunTag 24xGCN4 v1 peptide array to a mitochondrial targeting domain and mCherry |
| 60914 | pcDNA4TO-mito-mCherry-10xGCN4_v4 | A fusion protein of the SunTag10xGCN4 v4 peptide array to a mitochondrial targeting domain and mCherry |
| 61056 | pcDNA4TO-sfGFP-24xGCN4_v1 | A direct fusion of sfGFP to the 24xGCN4_v1 peptide array |
| 61057 | pcDNA4TO-24xGCN4_v1-P2A-sfGFP | sfGFP and the 24xGCN4_v1 peptide array are expressed from the same transcript, separated by a P2A site |
| 61058 | pcDNA4TO-24xGCN4_v4-sfGFP | A direct fusion of sfGFP to the 24xGCN4_v4 peptide array |
| 72228 | pcDNA4TO-Kif18b-24xGCN4_v1 | A fusion of the Kif18b motor protein to the v1 SunTag peptide array |
| 72229 | pHRdSV40-K560-24xGCN4_v4 | A fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4 SunTag peptide array |