Screening and large-scale expression of membrane proteins in mammalian cells for structural studies.
Goehring A, Lee CH, Wang KH, Michel JC, Claxton DP, Baconguis I, Althoff T, Fischer S, Garcia KC, Gouaux E
Nat Protoc. 2014 Nov;9(11):2574-85. doi: 10.1038/nprot.2014.173. Epub 2014 Oct 9. PubMed Article
Goehring A, Lee CH, Wang KH, Michel JC, Claxton DP, Baconguis I, Althoff T, Fischer S, Garcia KC, Gouaux E
Nat Protoc. 2014 Nov;9(11):2574-85. doi: 10.1038/nprot.2014.173. Epub 2014 Oct 9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160451 | pEG BacMam | vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein |
| 160680 | pEG BacMam N term His8 GFP | vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein |
| 160681 | pEG BacMam C term GFP His8 | Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein |
| 160682 | pEG BacMam N term myc eGFP 3C | Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein |
| 160683 | pEG BacMam N term StrepII eGFP 3C | Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein |
| 160684 | pEG BacMam N term His8 eGFP 3C | Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein |
| 160685 | pEG BacMam C term 3C eGFP myc | Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein |
| 160686 | pEG BacMam C term 3C eGFP StrepII | Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein |
| 160687 | pEG BacMam C term 3C eGFP His8 | Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein |