Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control.
Venkatachalam V, Cohen AE
Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. PubMed Article
Venkatachalam V, Cohen AE
Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58500 | VV063: 1xCox8 - superecliptic pHluorin in fck | Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells |
| 58502 | VV064: 1xCox8 - ratiometric pHluorin in fck | Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells |
| 58509 | VV073: 1xCox8 - GCaMP5G in fubi | Expresses the calcium sensor GCaMP5G in the mitochondria |
| 58510 | VV201: ChR2(C128S)-eGFP in fubi | Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter |
| 58512 | VV220: CoChR(C108S)-eGFP in fck | Expresses CoChR with the C108S (step function opsin) mutation fused to eGFP under the a-CamKII promoter |
| 58513 | VV221: sdChR(C138S)-TS-eGFP-ER in fck | Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter |
| 58514 | VV229: GCaMP6f in fck | Expresses GCaMP6f under the a-CamKII promoter |
| 58521 | VV246: CoChR(C108S)-GCaMP6f-ER in fck | Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells |
| 58522 | VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck | Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells |
| 58523 | VV228: sdChR(C138S E154A)-TS in fck | Expresses sdChR(C138S E154A) in mammalian cells |
| 58524 | VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck | Expresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cells |