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A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region.
Schroeder CM, Ostrem JM, Hertz NT, Vale RD
Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
74597pGEX6P1-human full-length LIC1used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag
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74598pGEX6P1-human LIC1 G domainused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389
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74599pGEX6P1-human LIC1 C-terminal halfused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523
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74600pET28a-RILPused for expression in E. coli and purification
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74601pHR-mCherry-p2A-full length LIC1expresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherry
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74602pHR-mCherry-p2A-LIC1 G domainexpresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherry
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74603pHR-mCherry-p2A-LIC1 C-terminal halfexpresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry
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74605pHR-mCherry-p2A-LIC1 Y101A, Y103Aexpresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein
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74606pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120Aexpresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein
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74753pET28a-FIP3Expressed sfGFP-tagged FIP3 in E. coli for protein production
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74780pGEX6P1-C. thermophilum LICencodes GST-tagged dynein light intermediate chain from Chaetomium thermophilum
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74782pET21a-Chaetomium thermophilum LICencodes dynein light intermediate chain (LIC) from Chaetomium thermophilum; no tag
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Antibodies from Article