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Designed Phosphoprotein Recognition in Escherichia coli.
Sawyer N, Gassaway BM, Haimovich AD, Isaacs FJ, Rinehart J, Regan L
ACS Chem Biol. 2014 Nov 21;9(11):2502-7. doi: 10.1021/cb500658w. Epub 2014 Oct 6. PubMed Article

Plasmids from Article

ID Plasmid Purpose
61965pNAS1B split mCherry N-link/link-CNegative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)
61966pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-CPositive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
61967pNAS1B split mCherry N-MESVD/TRAP(RK)-CDuet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD
61968pNAS1BDuet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

Antibodies from Article