An assay for clogging the ciliary pore complex distinguishes mechanisms of cytosolic and membrane protein entry.
Takao D, Dishinger JF, Kee HL, Pinskey JM, Allen BL, Verhey KJ
Curr Biol. 2014 Oct 6;24(19):2288-94. doi: 10.1016/j.cub.2014.08.012. Epub 2014 Sep 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 252747 | pN1_FV-NUP62-EGFP3 | Expression of NUP62 with three tandem EGFPs. FV is a modified FKBP domain (aka DmrB) whose dimerization is induced by addition of Rapalog2. Blocks nulcear pore in absence of Rapalog2. |
| 252748 | pN1_NUP62-EGFP3 | Expression of NUP62 with three tandem EGFPs. |
| 252749 | pN1_NUP62-Fv_EGFP3 | Expression of NUP62 with three tandem EGFPs. FV is a modified FKBP domain (aka DmrB) whose dimerization is induced by addition of Rapalog2. Blocks nulcear pore upon addition of Rapalog2 |
| 252750 | pN1_Rev-GR-EGFP | Plasmid for studying nuclear export. Expression of a fusion protein containing full-length HIV-1 Rev, followed by the hormone-responsive element of the rat glucocorticoid receptor (Gr), and GFP. |
| 252751 | pN1_Rev-GR(m10)-EGFP | Control for studying nuclear export. Expression of a fusion protein containing full-length Rev, the hormone-responsive element of the rat Gr, and GFP. The m10 mutation abolishes nuclear export. |