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The interaction of cytoplasmic poly(A)-binding protein with eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA decay.
Fatscher T, Boehm V, Weiche B, Gehring NH
RNA. 2014 Oct;20(10):1579-92. doi: 10.1261/rna.044933.114. Epub 2014 Aug 21. PubMed Article

Plasmids from Article

ID Plasmid Purpose
65807pCI-MS2V5-PABPC1expression of PABPC1-MS2 fusion. MS2 tethers PABPC1 to mRNAs containing MS2 stem loops.
65808pCI-MS2V5-PABPC1 MLLEmutexpression of PABPC1-MS2 fusion. Mutation of C-terminal MLLE domain.
65809pCI-MS2V5-PABPC1 M161A/D165Kexpression of PABPC1-MS2 fusion. Mutations abolish binding to eIF4G
65810pCI-MS2V5-PABPC1 MLLEmut M161A/D165Kexpression of PABPC1-MS2 fusion, double mutant.
65811pCI-MS2V5-eRF3a F76aexpression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1
65812pCI-MS2V5-eIF4G delN83expression of eIF4G-MS2 fusion.

Antibodies from Article