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Dynamic localization of the cyanobacterial circadian clock proteins.
Cohen SE, Erb ML, Selimkhanov J, Dong G, Hasty J, Pogliano J, Golden SS
Curr Biol. 2014 Aug 18;24(16):1836-44. doi: 10.1016/j.cub.2014.07.036. Epub 2014 Aug 7. PubMed Article

Plasmids from Article

ID Plasmid Purpose
85105pAM5084Ptrc-ECFP-KaiC fusion cloned into Km resistance gene of pACYC177. To look for KaiC localization in E. coli.
85106pAM5080Ptrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expres
85107pAM5081Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.
85121pAM5082PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp
85122pAM5085PkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.
85123pAM5087Ptrc-YFP subcloned from pAM5080 into NSII-Km vector.
85124pAM4645NSII vector expressing PkaiB-KaiBC. Nt resistant. Complements rhthyms in AMC705
85125pAM5089KaiB expressed from native promoter from NSII-Nt. Complements rhythms of kaiB null strain.

Antibodies from Article