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An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential.
Goodwin JM, Svensson RU, Lou HJ, Winslow MM, Turk BE, Shaw RJ
Mol Cell. 2014 Aug 7;55(3):436-50. doi: 10.1016/j.molcel.2014.06.021. Epub 2014 Jul 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
61210pENTR-FLAG-hDIXDC1-L-WTEntry clone encoding long isoform of human DIXDC1
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61211pENTR-FLAG-hDIXDC1-L-S592AEntry clone encoding long isoform of human DIXDC1
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61212pENTR-FLAG-hDIXDC1-L-F630SEntry clone encoding long isoform of human DIXDC1
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61213pBabe-Hygro-FLAG-hDIXDC1 WT Retrovirus expressing long isoform of human DIXDC1
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61214pBabe-Hygro-FLAG-hDIXDC1 S592ARetrovirus expressing long isoform of human DIXDC1
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61215pBabe-Hygro-GFPRetrovirus expressing GFP
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61216pDONR-mDIXDC1 WTEntry clone encoding long isoform of mouse DIXDC1
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61218pLenti-CMV/TO-Puro-FLAG-hDIXDC1 WTLentivirus expressing long isoform of human DIXDC1
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61219pLenti-CMV/TO-Puro-FLAG-hDIXDC1 S592ALentivirus expressing long isoform of human DIXDC1
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61220pLenti-PGK-Blast-FLAG-hDIXDC1 WTLentivirus expressing long isoform of human DIXDC1
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61221pLenti-PGK-Blast-FLAG-hDIXDC1 S592ALentivirus expressing long isoform of human DIXDC1
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61222pcDNA3-FLAG-hDIXDC1 WTMammalian expression vector encoding long isoform of human DIXDC1
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61223pcDNA3-FLAG-hDIXDC1 S592AMammalian expression vector encoding long isoform of human DIXDC1
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61224pQCXIB-FLAG-hDIXDC1-L-WTRetrovirus expressing long isoform of human DIXDC1
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61225pQCXIB-FLAG-hDIXDC1-L-S592ARetrovirus expressing long isoform of human DIXDC1
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61230pLentiX2-PURO-shGFPLentivirus expressing shRNA to GFP
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61231pLentiX2-PURO-shLKB1-MsLentivirus expressing shRNA to mouse LKB1
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61232pLentiX2-PURO-shDIXDC1-Ms-57Lentivirus expressing shRNA to mouse DIXDC1
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61233pLentiX2-PURO-shDIXDC1-Ms-58Lentivirus expressing shRNA to mouse DIXDC1
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61234pENTR-TER+-shLKB1-MsEntry clone encoding shRNA to mouse LKB1
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61235pENTR-TER+-shDIXDC1-Ms-57Entry clone encoding shRNA to mouse DIXDC1
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61236pENTR-TER+-shDIXDC1-Ms-58Entry clone encoding shRNA to mouse DIXDC1
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61237pENTR-TER+-shDIXDC1-Hu-16Entry clone encoding shRNA to human DIXDC1
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61239pENTR-MYC-hDIXDC1-L-WTEntry clone encoding long isoform of human DIXDC1
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61240pENTR-MYC-hDIXDC1-L-S592AEntry clone encoding long isoform of human DIXDC1
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61241pENTR-MYC-hDIXDC1-L-F630SEntry clone encoding long isoform of human DIXDC1
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61242pLentiX2-PURO-shLKB1-HuLentivirus expressing shRNA to human LKB1
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61243pENTR-TER+-shLKB1-HuEntry clone encoding shRNA to human LKB1
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Antibodies from Article