Probing the electrostatics of active site microenvironments along the catalytic cycle for Escherichia coli dihydrofolate reductase.

Liu CT, Layfield JP, Stewart RJ 3rd, French JB, Hanoian P, Asbury JB, Hammes-Schiffer S, Benkovic SJ
J Am Chem Soc. 2014 Jul 23;136(29):10349-60. doi: 10.1021/ja5038947. Epub 2014 Jul 11. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
110541	pET22b-ecDHFR-dCys	Bacterial protein of E.coli DHFR C85A/C152S mutant
110542	pET22b-ecDHFR-dCys-L54C	Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant
110543	pET22b-ecDHFR-dCys-T46C	Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant