Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase.
Kung Y, McAndrew RP, Xie X, Liu CC, Pereira JH, Adams PD, Keasling JD
Structure. 2014 Jul 8;22(7):1028-36. doi: 10.1016/j.str.2014.05.007. Epub 2014 Jun 19. PubMed Article
Kung Y, McAndrew RP, Xie X, Liu CC, Pereira JH, Adams PD, Keasling JD
Structure. 2014 Jul 8;22(7):1028-36. doi: 10.1016/j.str.2014.05.007. Epub 2014 Jun 19. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110148 | pSKB3SaGGR G91H (JBEI-3677) | SaGGR site-directed mutation of G91H |
| 110149 | pSKB3SaGGR I206F (JBEI-3678) | SaGGR site-directed mutation of I206F |
| 110150 | pSKB3SaGGR L377H (JBEI-3683) | SaGGR site-directed mutation of L377H |
| 110151 | pSKB3-SaGGR-F219L (JBEI-7186) | pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant gene |
| 110152 | pSKB3-SaGGR-I206F-L377H (JBEI-7202) | pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant gene |