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Chemogenetic synaptic silencing of neural circuits localizes a hypothalamus-->midbrain pathway for feeding behavior.
Stachniak TJ, Ghosh A, Sternson SM
Neuron. 2014 May 21;82(4):797-808. doi: 10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24. PubMed Article

Plasmids from Article

ID Plasmid Purpose
52520CAG:: ChR2HA-2a-hM4DCo-expresses HA tagged channelrhodopsin and hM4D
52521AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4DCo-expresses HA tagged channelrhodopsin and hM4D from a Cre-dependent virus
52523CAG:: mCherry-2a-hM4DnrxnCo-expresses hM4Dnrxn and a red fluorescent label
52525CMV:: HA-hM4DnrxnExpresses N-terminal HA tagged hM4Dnrxn
60544AA0250-mCherry-2a-hM4D-nrxn1a revCo-expresses hM4Dnrxn and a red fluorescent label from a Cre-dependent virus

Antibodies from Article