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A PKA activity sensor for quantitative analysis of endogenous GPCR signaling via 2-photon FRET-FLIM imaging.
Chen Y, Saulnier JL, Yellen G, Sabatini BL
Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. eCollection 2014.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
60445AAV-FLEX-FLIM-AKARExpresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone
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60446AAV-FLEX-FLIM-AKART391AExpresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backbone
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63058AAV-FLIM-AKARExpresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone
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63059AAV-FLEX-PKIalpha-IRES-nls-mRuby2Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone
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63060AAV-FLEX-tmeGFP-IRES-nls-mRuby2Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone
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Antibodies from Article