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SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture.

Fierer JO, Veggiani G, Howarth M
Proc Natl Acad Sci U S A. 2014 Mar 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
51722SpyLigasePlasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!
51723SUMO-KTagPlasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag

Antibodies from Article