Crystal structure of Cas9 in complex with guide RNA and target DNA.
Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O
Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58888 | PX543 SpCas9(del175-307) | Truncated form of SpCas9 without REC2 domain |
| 58889 | PX544 SpCas9(del1098-1368) | Truncated form of SpCas9 without C' domain |
| 68706 | PX429 SpCas9 N863A nickase | SpCas9 N863A nickase |