A Bicistronic MAVS Transcript Highlights a Class of Truncated Variants in Antiviral Immunity.
Brubaker SW, Gauthier AE, Mills EW, Ingolia NT, Kagan JC
Cell. 2014 Feb 13;156(4):800-11. doi: 10.1016/j.cell.2014.01.021. PubMed Article
Brubaker SW, Gauthier AE, Mills EW, Ingolia NT, Kagan JC
Cell. 2014 Feb 13;156(4):800-11. doi: 10.1016/j.cell.2014.01.021. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51999 | pcDNA3-HA-MAVS-M142A(KaganF61) | Expresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context" |
| 52000 | pcDNA3-MAVS(KaganF57) | Expresses the human MAVS CDS containing "weak translational context" |
| 52001 | pcDNA3-MAVS-M1A(KaganF58) | Expresses the human MAVS CDS containing the point mutation M1A |
| 52002 | pcDNA3-MAVS-M1A,M142A(KaganF62) | Expresses the human MAVS CDS containing two point mutations M1A and M142A |
| 52003 | pcDNA3-MAVS-M1A500(KaganF60) | Expresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domain |
| 52004 | pMSCV-HA-shift-IRES-GFP(KaganE21) | Expresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP marker |
| 52005 | pcDNA3-5'UTR-MAVS(KaganD88) | Expresses the human MAVS CDS and contains the 5'UTR sequense |
| 52006 | pcDNA3-5'UTR-MAVS-ORF1(KaganE49) | Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codon 37 nt upstream of the FL MAVS start codon |
| 52007 | pcDNA3-5'UTR-MAVS-ORF3,4(KaganE50) | Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codons 61 and 67 nt downstream of the FL MAVS start codon |
| 52008 | pMSCV-MAVS(KaganE17) | Expresses the human MAVS CDS |
| 52009 | pMSCV-MAVS-M1A(KaganE18) | Expresses the human MAVS CDS containing the point mutation M1A |
| 52010 | pMSCV-MAVS-M142A(KaganE19) | Expresses the human MAVS CDS containing the point mutation M142A |
| 52011 | pMSCV-MAVS-M1A,M142A(KaganE20) | Expresses the human MAVS CDS containing the point mutations M1A and M142A |
| 52012 | pcDNA3-MAVS-invitro(KaganD86) | Used for in vitro expression of the human MAVS CDS |
| 52013 | pcDNA3-MAVS-L62M-invitro(KaganD98) | Used for in vitro expression of the human MAVS CDS containing a point mutation L62M |
| 52014 | pcDNA3-MAVS-G67M-invitro(KaganD100) | Used for in vitro expression of the human MAVS CDS containing a point mutation G67M |
| 52015 | pcDNA3-MAVS-E80M-invitro(KaganD99) | Used for in vitro expression of the human MAVS CDS containing a point mutation E80M |
| 52016 | pcDNA3-MAVS-M1A-invitro(KaganE12) | Used for in vitro expression of the human MAVS CDS containing a point mutation M1A |
| 52017 | pcDNA3-MAVS-M142A-invitro(E13) | Used for in vitro expression of the human MAVS CDS containing a point mutation M142A |
| 52018 | pcDNA3-MAVS-M1A,M142A-invitro(KaganE14) | Used for in vitro expression of the human MAVS CDS containing the point mutations M1A,M142A |
| 52019 | p3XFlag-CMV7-miniMAVS(KaganE48) | Expresses miniMAVS (the sequence starting from M142 of the MAVS CDS) with an N-terminal 3X flag tag |
| 52062 | pcDNA3-MAVS-M142A(KaganF59) | Expresses the human MAVS CDS containing the point mutation M142A "weak translational context" |