Background free imaging of single mRNAs in live cells using split fluorescent proteins.

Wu B, Chen J, Singer RH
Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038/srep03615. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
52984	Pcr4-12xMBS-PBS	provide MBS-PBS alternating sequence
52985	ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC	split Venus attached to MCP and PCP respectively