The myeloma drug lenalidomide promotes the cereblon-dependent destruction of Ikaros proteins.
Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK, Bradner JE, Kaelin WG Jr
Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. Epub 2013 Nov 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69040 | pLKO-RFP-shCntrl | Lentiviral expression of control shRNA |
| 69041 | pLKO-RFP-IKZF1-sh2 | 3rd generation lentiviral vector expressing IKZF1 shRNA |
| 69042 | pLKO-RFP-IKZF1-sh3 | Lentiviral expression of IKZF1 shRNA |
| 69043 | pLKO-RFP-IKZF3-sh2 | 3rd generation lentiviral vector expressing IKZF3 shRNA |
| 69044 | pLKO-RFP-IKZF3-sh3 | 3rd generation lentiviral vector expressing IKZF3 shRNA |
| 69045 | pIRIGF-IKZF1-V2 | expression of IKZF1 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control. |
| 69046 | pIRIGF-IKZF3 | expression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control. |
| 69047 | plenti-CAG -IRES-GFP | Lentiviral backbone for GFP coexpression |
| 69048 | plenti-CAG-IKZF1-V1-Q146H-FLAG-IRES-GFP | lentiviral expression of IKZF1 variant 1 with Q146H mutation and FLAG tag |
| 69049 | plenti-CAG-IKZF2-V2-FLAG-IRES-GFP | lentiviral expression of IKZF2 variant 2 with FLAG tag |
| 69050 | plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP | lentiviral expression of IKZF3 with Q147H mutation and FLAG tag |
| 69051 | plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur | lentiviral expression of IKZF1 with HA tag |
| 69052 | plenti-UbcP-IKZF2-V2-3xHA-pGK-Pur | lentiviral expression of IKZF2 variant 2 with HA tag |
| 69053 | plenti-UbcP-IKZF3-3xHA-pGK-Pur | lentiviral expression of IKZF3 with HA tag |
| 101139 | pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF) | Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites. |
| 107374 | plenti-UbcP-FLAG-CRBN-pGK-HYG | Lentiviral expression of FLAG-CRBN |
| 107375 | plenti-UbcP-FLAG-YWAA-pGK-HYG | Lentiviral expression of FLAG-YWAA |
| 107376 | plenti-UbcP-Myc-CRBN-pGK-HYG | Lentiviral expression of myc-CRBN |
| 107377 | plenti-UbcP-Myc-YWAA-pGK-HYG | Lentiviral expression of myc-YWAA |
| 107378 | plenti-UbcP-HA-CRBN-pGK-HYG | Lentiviral expression of HA-CRBN |
| 107379 | plenti-UbcP-HA-YWAA-pGK-HYG | Lentiviral expression of HA-YWAA |
| 107380 | pcDNA3-FLAG-CRBN-pGK-HYG | Mammalian expression of FLAG-CRBN |
| 107381 | pcDNA3-FLAG -YWAA-pGK-HYG | Mammalian expression of FLAG-YWAA |
| 107382 | plenti-px330-CRBN-T1-pGK-Pur | Mammalian expression CRISPR/Cas9 |
| 107383 | plenti-px330-CRBN-T2-pGK-Pur | Mammalian expression CRISPR/Cas9 |
| 107384 | plenti-UBC- IKZF4-3xHA-pGK-PUR | Lentiviral expression of IKZF4-3xHA |
| 107385 | plenti-UBC- IKZF5-3xHA-pGK-PUR | Lentiviral expression of IKZF5-3xHA |
| 107386 | plenti-UBC- IRF4-3xHA-pGK-PUR | Lentiviral expression of IRF4-3xHA |
| 107387 | plenti-CAG-IKZF1-V1-FLAG-IRES-GFP | Lentiviral expression of IKZF1-V1-FLAG |
| 107388 | plenti-CAG-IKZF3-FLAG-IRES-GFP | Lentiviral expression of IKZF3-FLAG |
| 107389 | plenti-CAG-IRF4-FLAG-IRES-GFP | Lentiviral expression of IRF4-FLAG |
| 107390 | pcDNA3-IKZF1-V2-V5 | Mammalian expression of IKZF1-V2-V5 |
| 107391 | pcDNA3-IKZF2-V2-V5 | Mammalian expression of IKZF2-V2-V5 |
| 107392 | pCMV-8xHis-Ub | Mammalian expression 8xHis-Ub |
| 107393 | plenti-UBC-gate-3xHA-pGK-PUR | Lentiviral gateway destination vector |
| 107395 | plenti-UBC-Myc-gate-pGK-HYG | Lentiviral gateway destination vector |
| 107396 | plenti-UBC-HA-gate-pGK-HYG | Lentiviral gateway destination vector |
| 107397 | pcDNA3-FLAG- gate-pGK-HYG | Gateway destination vector |
| 107398 | plenti-CAG-gate-FLAG-IRES-GFP | Lentiviral gateway destination vector |