megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering.
Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
Nucleic Acids Res. 2013 Nov 26. PubMed Article
Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
Nucleic Acids Res. 2013 Nov 26. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 50420 | pCVL TAL(Nd154C63) SFFV Xba-Sal linker | pTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites. |
| 50421 | pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers | pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites. |
| 50422 | pFUSC2 | Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 2.5 RVDs long. |
| 50423 | pFUSC3 | Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 3.5 RVDs long. |
| 50424 | pFUSC4 | Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 4.5 RVDs long. |
| 50425 | pFUSC5 | Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 5.5 RVDs long. |
| 50426 | pFUSC6 | Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 6.5 RVDs long. |
| 50427 | pFUSC7 | Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 7.5 RVDs long. |
| 50428 | pFUSC8 | Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 8.5 RVDs long. |
| 50429 | pFUSC9 | Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 9.5 RVDs long. |
| 50430 | pFUSC10 | Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 10.5 RVDs long. |
| 50434 | pCVL SFFV Sbf-Sal linker | Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal). |