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Deterministic direct reprogramming of somatic cells to pluripotency.
Rais Y, Zviran A, Geula S, Gafni O, Chomsky E, Viukov S, Mansour AA, Caspi I, Krupalnik V, Zerbib M, Maza I, Mor N, Baran D, Weinberger L, Jaitin DA, Lara-Astiaso D, Blecher-Gonen R, Shipony Z, Mukamel Z, Hagai T, Gilad S, Amann-Zalcenstein D, Tanay A, Amit I, Novershtern N, Hanna JH
Nature. 2013 Oct 3;502(7469):65-70. doi: 10.1038/nature12587. Epub 2013 Sep 18.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
52356FUW-Mbd3Lentiviral expression vector for constitutive expression of Mbd3
523585' MBD3 TALENTALEN vector for targeting human MBD3 locus (5')
523593' MBD3 TALENTALEN vector for targeting human MBD3 locus (3')
52362pNTK human MBD3 PGK-Neo-pA floxTargeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.
52371FUW-Flag-Mbd3Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3
52372FUW-Flag-Mbd3 delta1-70Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant
52373FUW-Flag-Mbd3 delta 25-83Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant
52374FUW-Flag-Mbd3 delta49-63 Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant
52376pBRPy CAGGS-Mbd3-IRES-PUROMammalian expression vector for constitutive expression of Mbd3
52409pBRY-nuclear mCherry-IRES-PUROConstitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.
52416FUW-CAGGS-ERASLentiviral vector encoding constitutive expression of human ERAS cDNA
52417FUW-TetO-lox-ERASDoxycycline inducible lentiviral vector of human ERAS cDNA with excisable insert

Antibodies from Article