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CHD5 is required for neurogenesis and has a dual role in facilitating gene expression and polycomb gene repression.
Egan CM, Nyman U, Skotte J, Streubel G, Turner S, O'Connell DJ, Rraklli V, Dolan MJ, Chadderton N, Hansen K, Farrar GJ, Helin K, Holmberg J, Bracken AP
Dev Cell. 2013 Aug 12;26(3):223-36. doi: 10.1016/j.devcel.2013.07.008.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
68869pENTR3c GW CHD5GW entry vector for CHD5
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68870pCMV-FLAG-CHD5-WT-331-672aaMammalian expression of CHD5 histone binding domain (331-672aa)
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68871pCMV-FLAG-CHD5-Y619E-FRAGMammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation
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68873pGEX-GST-CHD5-WT-FRAGIPTG inducible bacterial expression of CHD5 fragment
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68874pGEX-GST-CHD5-L518A/Y619E-FRAGIPTG inducible bacterial expression of CHD5 fragment
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68875pGEX-GST-CHD5(Chromos)-FRAGIPTG inducible bacterial expression of CHD5 fragment
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68876pAAV-MCS-shCHD5 #1CHD5 shRNA expressed from Adeno-associated viral (AAV) vector
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68877pAAV-MCS-shCHD5 #2CHD5 shRNA expressed from Adeno-associated viral (AAV) vector
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68878pCAG-Flag-Avi-ires-CHD5Transient mammalian expression of CHD5 for BirA tagging
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68879pCR8-CHD5-FRAGMENTGW entry vector for CHD5
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Antibodies from Article