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Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR.
Fujita T, Fujii H
Biochem Biophys Res Commun. 2013 Sep 13;439(1):132-6. doi: 10.1016/j.bbrc.2013.08.013. Epub 2013 Aug 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
479483xFLAG-dCas9/pCMV-7.1Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
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61079gRNA-hIRF-1 #12Expresses a guide RNA (gRNA) to target human IRF-1 promoter
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Antibodies from Article