CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang L, Church GM
Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. PubMed Article
Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang L, Church GM
Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47314 | Cas9m2 | Cas9 D10A+H840A |
| 47315 | Cas9m3 | Cas9 D10A+D839A+H840A |
| 47316 | Cas9m4 | Cas9 D10A+D839A+H840A+N863A |
| 47317 | Cas9m2-VP64 | Cas9m2 Activator |
| 47318 | Cas9m3-VP64 | Cas9m3 Activator |
| 47319 | Cas9m4-VP64 | Cas9m4 Activator |
| 47320 | reporter-gT1 | TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression) |
| 47321 | reporter-gT2 | TF reporter for gRNA-AAVS1_T2 (RNA-guided dTomato expression) |