Functional significance of evolving protein sequence in dihydrofolate reductase from bacteria to humans.
Liu CT, Hanoian P, French JB, Pringle TH, Hammes-Schiffer S, Benkovic SJ
Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10159-64. doi: 10.1073/pnas.1307130110. Epub 2013 Jun 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118582 | pET22b-ecDHFR-G51PEKN | Bacterial expression of E.coli DHFR G51PEKN mutant |
| 118583 | pET22b-ecDHFR-N23PP/G51PEKN | Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant |
| 118584 | pET27b-ecDHFR-N23PP/L28F | Bacterial expression of E.coli DHFR N23PP and L28F mutant |
| 118585 | pET22b-ecDHFR-N23PP/L28F/G51PEKN | Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant |