Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3.
Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith A
Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48753 | pPB-CAG-empty-pgk-hph | PiggyBac backbone, CAG promoter, Hygromycin selection |
| 48754 | pPB-CAG-3xFLAG-empty-pgk-hph | PiggyBac backbone, CAG promoter, Hygromycin selection, N-terminal 3xFLAG tag |
| 48755 | pPB-CAG-Tfe3-pgk-hph | PiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin selection |
| 48756 | pPB-CAG-Tfe3::ERT2-pgk-hph | PiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, Hygromycin selection |
| 48757 | pPB-CAG-3xFLAG::Flcn-pgk-hph | PiggyBac backbone, CAG promoter driving murine 3xFLAG-Flcn fusion, Hygromycin selection |
| 48758 | pPB-CAG-3xFLAG::Fnip1-pgk-hph | PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selection |
| 48759 | pPy-CAG-GFP-IRES-Pac | CAG promoter driving GFP-IRES-Puromycin Acetyl Transferase |
| 48760 | pPy-CAG-Cre::ERT2-IRES-BSD | CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance |