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Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells.
Holland AJ, Fachinetti D, Han JS, Cleveland DW
Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 10.1073/pnas.1216880109. Epub 2012 Nov 12.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
47328pBabe TIR1-9mycFor retroviral expression of E3 ubiquitin ligase osTIR1 with N-term NES and C-term 9x myc tag
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47329pcDNA5-H2B-AID-EYFPfor plasmid integration using the Flp-In system. AID at C-terminal
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47330pcDNA5-EGFP-AID-BubR1for plasmid integration using the Flp-In system. AID at N-terminal
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64945pBABE TIR1-9mycFor retroviral expression of E3 ubiquitin ligase osTIR1 with 9x myc tag
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Antibodies from Article