Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy.
Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY
Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375. PubMed Article
Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY
Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 40306 | pUC57 APEX | A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386). |
| 40307 | pCAG APX W41F dimeric (mito matrix) | |
| 40863 | pcDNA3 tdAPX W41F | Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386). |
| 44439 | pcDNA3 Connexin43-APEX | |
| 44440 | pcDNA3 Connexin43-GFP-APEX |