Novel insights from hybrid LacI/GalR proteins: family-wide functional attributes and biologically significant variation in transcription repression.
Meinhardt S, Manley MW Jr, Becker NA, Hessman JA, Maher LJ 3rd, Swint-Kruse L
Nucleic Acids Res. 2012 Nov;40(21):11139-54. doi: 10.1093/nar/gks806. Epub 2012 Sep 10. PubMed Article
Meinhardt S, Manley MW Jr, Becker NA, Hessman JA, Maher LJ 3rd, Swint-Kruse L
Nucleic Acids Res. 2012 Nov;40(21):11139-54. doi: 10.1093/nar/gks806. Epub 2012 Sep 10. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 90040 | LLhS-pHG165a | Constitutive expression of a chimeric LacI:GalS transcription repressor |
| 90041 | LLhF-pHG165a | Constitutive expression of a chimeric LacI:FruR transcription repressor |
| 90042 | LLhT-pHG165a | Constitutive expression of a chimeric LacI:TreR transcription repressor |
| 90043 | LLhR-pHG165a | Constitutive expression of a chimeric LacI:RbsR transcription repressor |
| 90044 | LLhA-pHG165a | Constitutive expression of a chimeric LacI:AscG transcription repressor |
| 90045 | LLhE-pHG165 | Constitutive expression of a chimeric LacI:CelR transcription repressor |
| 90046 | LLhC-pHG165a | Constitutive expression of a chimeric LacI:CytR transcription repressor |
| 90050 | LLhT-pHG165a V52A | Constitutive expression of a chimeric LacI:TreR transcription repressor |
| 90052 | LLhA-pHG165a Q55A | Constitutive expression of a chimeric LacI:AscG transcription repressor |
| 90053 | LLhC-pHG165a Q55I | Constitutive expression of a chimeric LacI:CytR transcription repressor |
| 90058 | LacI/109-pHG165c | WT LacI polymorphism with T at position 109 |
| 90059 | LacI-11/109-pHG165c | Dimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109 |
| 90062 | LLhE_AW-pHG165 | Constitutive expression of a chimeric LacI:CelR transcription repressor |
| 90064 | DEL-pHG165a | LLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmid |