Improving FRET dynamic range with bright green and red fluorescent proteins.
Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ
Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. PubMed Article
Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ
Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 40255 | pcDNA3-AKAR2-CR | |
| 40256 | pcDNA3-Camui-CR | |
| 40257 | pcDNA3.1/Puro-CAG-VSFP-CR | |
| 40258 | pCAGGS-Raichu-RhoA-CR | |
| 40259 | pcDNA3-Clover | GFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap |
| 40260 | pcDNA3-mRuby2 | RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap |
| 54551 | Clover-Lifeact-7 | Localization: Actin, Excitation: 505, Emission: 515 |
| 54561 | mRuby2-NT-C1 | Localization: C1 Cloning Vector, Excitation: 559, Emission: 600 |
| 54575 | Clover-pBAD | Localization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016. |
| 54612 | mRuby2-NT-N1 | Localization: N1 Cloning Vector, Excitation: 559, Emission: 600 |
| 54614 | mRuby2-N1 | Localization: N1 Cloning Vector, Excitation: 559, Emission: 600 |
| 54674 | mRuby2-Lifeact-7 | Localization: Actin, Excitation: 559, Emission: 600 |
| 54768 | mRuby2-C1 | Localization: C1 Cloning Vector, Excitation: 559, Emission: 600 |
| 54771 | mRuby2-pBAD | Localization: Protein Expression Vector , Excitation: 559, Emission: 600 |
| 55888 | mRuby2-Actin-7 | Localization: Actin, Excitation: 559, Emission: 600 |
| 55889 | mRuby2-Actin-C-18 | Localization: Actin, Excitation: 559, Emission: 600 |
| 55890 | mRuby2-Alpha-Actinin-19 | Localization: Alpha Actinin, Excitation: 559, Emission: 600 |
| 55891 | mRuby2-Calnexin-N-14 | Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600 |
| 55892 | mRuby2-Calreticulin-N-16 | Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600 |
| 55893 | mRuby2-CENPB-N-22 | Localization: Nucleus/Centromeres, Excitation: 559, Emission: 600 |
| 55894 | mRuby2-Cx43-7 | Localization: Gap Junctions, Excitation: 559, Emission: 600 |
| 55895 | mRuby2-CytERM-N-17 | Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600 |
| 55896 | mRuby2-Farnesyl-5 | Localization: Membrane, Excitation: 559, Emission: 600 |
| 55897 | mRuby2-H2B-6 | Localization: Nucleus/Histones, Excitation: 559, Emission: 600 |
| 55898 | mRuby2-H2B-C-10 | Localization: Nucleus/Histones, Excitation: 559, Emission: 600 |
| 55899 | mRuby2-JUP-C-14 | Localization: Tight Junctions, Excitation: 559, Emission: 600 |
| 55900 | mRuby2-Keratin-17 | Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 559, Emission: 600 |
| 55901 | mRuby2-LaminA-C-18 | Localization: Nuclear Envelope, Excitation: 559, Emission: 600 |
| 55902 | mRuby2-Lysosomes-20 | Localization: Lysosome Membrane, Excitation: 559, Emission: 600 |
| 55903 | mRuby2-MannII-N-10 | Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600 |
| 55904 | mRuby2-MAPTau-C-10 | Localization: Microtubules, Excitation: 559, Emission: 600 |
| 55905 | mRuby2-Mito-7 | Localization: Mitochondria, Excitation: 559, Emission: 600 |
| 55906 | mRuby2-MyosinIIA-C-18 | Localization: Cytoskeleton, Excitation: 559, Emission: 600 |
| 55907 | mRuby2-Myotilin-C-14 | Localization: Cytoskeleton, Excitation: 559, Emission: 600 |
| 55908 | mRuby2-Nup50-N-10 | Localization: Nuclear Pore, Excitation: 559, Emission: 600 |
| 55909 | mRuby2-PDHA1-N-10 | Localization: Mitochondria, Excitation: 559, Emission: 600 |
| 55910 | mRuby2-PMP-N-10 | Localization: Peroxisomes, Excitation: 559, Emission: 600 |
| 55911 | mRuby2-Rab5a-7 | Localization: Ras GTPase, Excitation: 559, Emission: 600 |
| 55912 | mRuby2-SiT-N-15 | Localization: Golgi TGN, Excitation: 559, Emission: 600 |
| 55913 | mRuby2-TOMM20-N-10 | Localization: Outer Mitochondrial Membrane, Excitation: 559, Emission: 600 |
| 55914 | mRuby2-Tubulin-6 | Localization: Microtubules, Excitation: 559, Emission: 600 |
| 55915 | mRuby2-Tubulin-C-18 | Localization: Microtubules, Excitation: 559, Emission: 600 |
| 55916 | mRuby2-ZO1-C-14 | Localization: Tight Junctions, Excitation: 559, Emission: 600 |
| 55917 | mRuby2-Zyxin-6 | Localization: Focal Adhesions, Excitation: 559, Emission: 600 |
| 56301 | Clover-CytERM-N-17 | Localization: Endoplasmic Reticulum, Excitation: 505, Emission: 515 |
| 56302 | Clover-MannII-N-10 | Localization: Mannosidase/Golgi, Excitation: 505, Emission: 515 |
| 56303 | Clover-Nup50-N-10 | Localization: Nuclear Pore, Excitation: 505, Emission: 515 |
| 56304 | Clover-PDHA1-N-10 | Localization: Mitochondria, Excitation: 505, Emission: 515 |
| 56305 | Clover-PMP-N-10 | Localization: Peroxisomes, Excitation: 505, Emission: 515 |
| 56306 | Clover-SiT-N-15 | Localization: Golgi TGN, Excitation: 505, Emission: 515 |
| 56307 | Clover-TOMM20-N-10 | Localization: Outer Mitochondrial Membrane, Excitation: 505, Emission: 515 |
| 56370 | Clover-LaminA-C-18 | Localization: Nuclear Envelope, Excitation: 505, Emission: 515 |
| 56371 | Clover-MAPTau-C-10 | Localization: Microtubules, Excitation: 505, Emission: 515 |
| 56372 | Clover-MyosinIIA-C-18 | Localization: Cytoskeleton, Excitation: 505, Emission: 515 |
| 56373 | Clover-Myotilin-C-14 | Localization: Cytoskeleton, Excitation: 505, Emission: 515 |
| 56374 | Clover-Tubulin-C-18 | Localization: Microtubules, Excitation: 505, Emission: 515 |
| 56375 | Clover-ZO1-C-14 | Localization: Tight Junctions, Excitation: 505, Emission: 515 |
| 56522 | Clover-Cx43-7 | Localization: Gap Junctions, Excitation: 505, Emission: 515 |
| 56528 | Clover-Lysosomes-20 | Localization: Lysosome Membrane, Excitation: 505, Emission: 515 |
| 56529 | Clover-Mito-7 | Localization: Mitochondria, Excitation: 505, Emission: 515 |
| 56530 | Clover-Rab5a-7 | Localization: Ras GTPase, Excitation: 505, Emission: 515 |
| 56531 | Clover-Zyxin-6 | Localization: Focal Adhesions, Excitation: 505, Emission: 515 |