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Endoplasmic reticulum stress-responsive transcription factor ATF6alpha directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes.
Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, Conaway RC
J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 10.1074/jbc.M112.369504. Epub 2012 May 10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
44150pGEM-HSPA5Pa fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)
64771pcDNA5-Flag-Med25Mammalian protein expression of flag-tagged Med25
64772pcDNA5-Flag-Med25-VWAMammalian expression of Flag-tagged Med25-VWA
64773pET21a-Med25-VBD-6HisBacterial expression of His-tagged Med25-VBD
64774pET41s-GST-ATF6a-ADbacterial expression of GST-tagged ATF6 (1-150)

Antibodies from Article