Regulation of nuclear PKA revealed by spatiotemporal manipulation of cyclic AMP.
Sample V, DiPilato LM, Yang JH, Ni Q, Saucerman JJ, Zhang J
Nat Chem Biol. 2012 Feb 26;8(4):375-82. doi: 10.1038/nchembio.799.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138214 | pcDNA3-sACt-mCherry-NLS | Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted. |
| 138215 | pcDNA3-sACt-mCherry-NES | Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted. |
| 138216 | pcDNA3-Lyn-sACt-mCherry | Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted. |
| 138217 | pcDNA3-AKAR4-NLS | Nuclear-targeted FRET-based PKA activity reporter. |
| 138218 | pcDNA3-ICUE3-NLS | Nuclear-targeted FRET-based cAMP indicator. |