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Structure of the Bro1 domain protein BROX and functional analyses of the ALIX Bro1 domain in HIV-1 budding.
Zhai Q, Landesman MB, Robinson H, Sundquist WI, Hill CP
PLoS One. 2011;6(12):e27466. doi: 10.1371/journal.pone.0027466. Epub 2011 Dec 5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
89602pet151D-TOPO BROX 1-411; Expresses full length human Brox in bacteria; internal ID WISP11-297
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89859pCINeoFlag ALIXExpresses full length Flag-tagged ALIX in mammalian cells; internal ID WISP03-308
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89861pCINeoFlag ALIX; I212DExpresses full length Flag-tagged ALIX with mutation I212D in mammalian cells; internal ID WISP05-117
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89864pet151D-TOPO-BROX 1-411; L212DExpresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300
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89865pet151D-TOPO-BROX 1-411; K141EExpresses full length human BROX with mutation K141E in bacteria; internal ID WISP11-299
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89866pet151D-TOPO-BROX 1-401Expresses human BROX residues 1-401 in bacteria; internal ID WISP11-297
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89867pCINeoFlag ALIX; I318AExpresses full length Flag-tagged ALIX with mutation I318A in mammalian cells; internal ID WISP11-403
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Antibodies from Article