miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs.
Chekulaeva M, Mathys H, Zipprich JT, Attig J, Colic M, Parker R, Filipowicz W
Nat Struct Mol Biol. 2011 Oct 7;18(11):1218-26. doi: 10.1038/nsmb.2166. PubMed Article
Chekulaeva M, Mathys H, Zipprich JT, Attig J, Colic M, Parker R, Filipowicz W
Nat Struct Mol Biol. 2011 Oct 7;18(11):1218-26. doi: 10.1038/nsmb.2166. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92251 | pMCh-466 | Plasmid for expression of GST-tagged human TNRC6C CED in mammalian cells |
| 92252 | pMCh-664 | Plasmid for expression of GST-tagged human TNRC6C CED with mutated PAM2 motif in mammalian cells |
| 92253 | pMCh-633 | Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs in mammalian cells |
| 92254 | pMCh-665 | Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells |
| 92255 | pMCh-1016 | Plasmid for expression of HA-tagged human TNRC6C CED in mammalian cells |
| 92256 | pMCh-133 | Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells |
| 92257 | pMCh-658 | Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells with mutated PAM2 motif |
| 92258 | pMCh-607 | Plasmid for expression of NHA-tagged human TNRC6C CED 7W7A with seven mutated W-motifs in mammalian cells |
| 92259 | pMCh-660 | Plasmid for expression of NHA-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells |
| 92263 | pMCh-695 | Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells |
| 92266 | pMCh-623 | Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells |
| 92289 | pMCh-646 | Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells |