A rapid and robust method for selective isotope labeling of proteins.
Lin MT, Sperling LJ, Frericks Schmidt HL, Tang M, Samoilova RI, Kumasaka T, Iwasaki T, Dikanov SA, Rienstra CM, Gennis RB
Methods. 2011 Dec;55(4):370-8. doi: 10.1016/j.ymeth.2011.08.019. Epub 2011 Sep 8. PubMed Article
Lin MT, Sperling LJ, Frericks Schmidt HL, Tang M, Samoilova RI, Kumasaka T, Iwasaki T, Dikanov SA, Rienstra CM, Gennis RB
Methods. 2011 Dec;55(4):370-8. doi: 10.1016/j.ymeth.2011.08.019. Epub 2011 Sep 8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61906 | ML2 | C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. ilvE gene deleted. |
| 61907 | ML3 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG gene deleted. |
| 61908 | ML6 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. ilvE, avtA genes deleted. |
| 61909 | ML8 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. argH gene deleted. |
| 61910 | ML12 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. ilvE avtA aspC genes deleted. |
| 61911 | ML14 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted. |
| 61912 | ML17 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. glnA gene deleted. |
| 61914 | ML21 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG tyrA genes deleted. |
| 61915 | ML24 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG asnA genes deleted. |
| 61916 | ML25 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG asnA asnB genes deleted. |
| 61917 | ML26 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH genes deleted. |
| 61918 | ML31 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA genes deleted. |
| 61919 | ML36 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG metA genes deleted. |
| 61920 | ML40K1 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA lysA genes. deleted. Contains pACYC plasmid. |
| 61921 | ML42 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA lysA thrC asnB genes deleted. |
| 61922 | ML43 | C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA lysA thrC asnA asnB genes deleted. |