Construction and evaluation of pMycoFos, a fosmid shuttle vector for Mycobacterium spp. with inducible gene expression and copy number control.
Ly MA, Liew EF, Le NB, Coleman NV
J Microbiol Methods. 2011 Sep;86(3):320-6. doi: 10.1016/j.mimet.2011.06.005. Epub 2011 Jun 13. PubMed Article
Ly MA, Liew EF, Le NB, Coleman NV
J Microbiol Methods. 2011 Sep;86(3):320-6. doi: 10.1016/j.mimet.2011.06.005. Epub 2011 Jun 13. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84577 | pMycoFos | Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli. |