The essential Ubc4/Ubc5 function in yeast is HECT E3-dependent, and RING E3-dependent pathways require only monoubiquitin transfer by Ubc4.

Stoll KE, Brzovic PS, Davis TN, Klevit RE
J Biol Chem. 2011 Apr 29;286(17):15165-70. Epub 2011 Feb 25. (Link opens in a new window) PubMed (Link opens in a new window) Article

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