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A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging.
Booth DG, Hood FE, Prior IA, Royle SJ
EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4. PubMed Article

Plasmids from Article

ID Plasmid Purpose
59355pBrain-GFP-shTACC3Knocks down TACC3 in mammalian cells via shRNA and co-expresses GFP.
59356pBrain-GFP-TACC3KDP-shTACC3Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.
59357pBrain-GFP-TACC3KDP(S558A)-shTACC3Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.
59799GFP-CHC17KDPExpresses knockdown-proof human CHC17 tagged with GFP.
60004pBrain-GFP-shGL2Co-expresses control GL2 shRNA and GFP.

Antibodies from Article